High-performance Liquid Chromatography - Wikipedia
High-performance liquid chromatography (HPLC; The time at which a specific analyte elutes (emerges from the column) is called its retention time. The retention time measured under particular conditions is an identifying characteristic of a given analyte. ... Read Article
Retention Time Changes - Alfresco.ubm-us.net
LC TROUBLESHOOTING The retention time of a peak in a liquid chromatography (LC) separation can be a all peaks should shift approximately the same, but if the bubble problem Changes in retention time can be a key to tracking down LC problems. ... Get Content Here
Final TIPS And Tricks HPLC Troubleshooting - Agilent
Tips and Tricks of HPLC System Troubleshooting Agilent Technologies, Inc. LC Tips And Tricks Seminar Series C. Retention. 3 Page 5 Column Observations Potential Problems High pressure - Plugged frit This Is Time Consuming Often Performed Offline Page 8 ... View This Document
Using A Checklist Approach To Troubleshoot Retention Time ...
To Troubleshooting HPLC retention time irreproducibility. May / June 2015 54 problems when retention times shift. troubleshooting a retention time issue then flow rate would be the first consideration. b) No change – suspect a problem with %B or temperature ... Access This Document
GPC/SEC TroubleshooTing - PSS USA
• elution Volume/retention time shift • slow Pressure built-uP after column (re-)installment GPC/SEC TroubleshooTing In collaboration with Volume 1. GPC/SEC Troubleshooting PSS Compendium www.pss-polymer.com IntroduCtIon In contrast to HPLC, broad sample peaks in GPC/SEC are quite common. ... Fetch Content
Gas Chromatography Troubleshooting And Reference Guide
Troubleshooting information special to a particular model of gas chro matograph. Performance specifica tions are often contained in the manuals. Eight Problem Categories Most performance problems can be placed within one of eight areas. These are baseline distur bances, irregular peak shapes or sizes, retention time shifts, loss of ... Access Document
Retention Time Vs Peak Area - Waters Corporation
Peak retention time is the first level of peak identification need for high performance HPLC equipment to assure reproducible highest level of performance? Because their results (quantitation and calibration) are directly linked to retention time reproducibility. Retention Time (min) Set ... Read More
LC Chromatography Troubleshooting - LCGCINDIA
LC Chromatography Troubleshooting Are certified by HPLC analysis for low extractables and include a certificate of analysis. In this case you will typically see the peak retention time shift slightly shorter. This is also the simplest to ... Return Doc
An Introduction To Elevated Temperature In HPLC
An Introduction to Elevated Temperature in HPLC decrease in retention. Additionally, decreased solvent viscosity at elevated temperature (1-2% per °C) leads to lower back pressure. programming the temperature in time can be used to elute compounds from the ... View Full Source
Troubleshooting Common System Problems Using Waters Neutrals ...
ACQUITY UPLC Columns, HPLC, Troubleshooting When a minor leak was present in the pump, all of the peaks were still eluting within the sample run time; however, there is a shift in retention times and a slight change in the system pressure. With stringent ... View Document
HPLC Troubleshooting Guide
6 www.ace-hplc.com Introduction This troubleshooting guide contains examples of some of the most common problems observed in reversed-phase HPLC (RP-HPLC) separations. Four major problem areas are covered: peak shape, retention time changes, ghost peaks and problems related to column backpressure. In addition, a section on column care is ... Get Document
HPLC Troubleshooting Cover - CCC/UPCMLD
HPLC Troubleshooting Uwe D. Neue, Waters Corporation 1. Column Life-Time 2. Variable Retention Times 3. Drifting Retention Times change of as little as 0.1 pH units can result in a retention time shift of 10%. So it is very important to measure the pH accurately and to keep the pH meter well ... Doc Viewer
Successful HPLC Operation - Troubleshooting Guide
A Troubleshooting Guide – Version 1.1. 2 1. Introduction 4 1.1 Purpose 4 3.4.1 Retention Time Changes from Injection to Injection 14 each section are included for rapid troubleshooting and less experienced HPLC users. ... Retrieve Document
LC Troubleshooting Series Retention Time Shifts - Agilent
LC Troubleshooting Series . Retention Time Shifts . Introduction . John Henderson is an Applications Scientist for Agilent Chemistries and Supplies Division. Maureen Joseph is the HPLC Columns Product Manager. Some possible causes to consider for retention time changes are: • Column aging • Column contamination ... Retrieve Doc
The Theory Of HPLC Chromatographic Parameters
Retention time of uracil (reversed phase HPLC) Retention time of hexane (normal phase HPLC) Retention factor is independent of some key variable factors including small flow rate variations and column dimensions. Therefore, it is a useful parameter when comparing retention of chromatographic peaks obtained using different HPLC systems. ) ... Fetch This Document
HPLC Troubleshooting - Proteins And Proteomics
HPLC Troubleshooting 1. INTRODUCTION Locating and Correcting the Problem Prevention 2. ABNORMAL PRESSURE J. Retention Time Drifts K. Abrupt Retention Time Changes L. Baseline Drift record the incident in the HPLC system record book to help with future problems. Prevention ... Document Retrieval
Internal Standard - Wikipedia
An internal standard in analytical chemistry is a chemical substance that is added in a constant amount to samples, This relative difference to TMS is called chemical shift, The internal standard selected should be again similar to the analyte and have a similar retention time and ... Read Article
Untangle Your Liquid Chromatography Problems HPLC ...
HPLC Troubleshooting Guide With increasing column life, the retention times may shift towards less retentivity, especially if the user is working at acidic pH (≤ pH 2). Abrupt changes in Retention time too long • Use gradient elution or stronger ... Retrieve Doc
A Guide To The Analysis And Purification Of Proteins ... - HPLC
A Guide to the Analysis and Purification of Proteins and Peptides by at which time the protein desorbs from the surface and elutes from the molecules in reversed-phase HPLC. While small molecules change retention slowly ... Return Doc
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